testpack® +plustm hcg urine Search Results


testpack® +plustm hcg urine  (Abbott Laboratories)
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Abbott Laboratories testpack® +plustm hcg urine
Testpack® +Plustm Hcg Urine, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g1-plustm sequential  (Vitrolife Inc)
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Vitrolife Inc g1-plustm sequential
G1 Plustm Sequential, supplied by Vitrolife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptflc3 plasmid mrfp-gfp-lc3  (Addgene inc)
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Addgene inc ptflc3 plasmid mrfp-gfp-lc3
Ptflc3 Plasmid Mrfp Gfp Lc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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steadylite plustm promega  (Promega)
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Promega steadylite plustm promega
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Steadylite Plustm Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lipofectaminetm ltx with plustm reagent  (Fisher Scientific)
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Fisher Scientific lipofectaminetm ltx with plustm reagent
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Lipofectaminetm Ltx With Plustm Reagent, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plustm reagent  (Thermo Fisher)
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Thermo Fisher plustm reagent
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Plustm Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spectrafluor plustm  (Tecan Systems)
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Tecan Systems spectrafluor plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Spectrafluor Plustm, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g1-plustm sequential medium  (Vitrolife Inc)
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Vitrolife Inc g1-plustm sequential medium
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
G1 Plustm Sequential Medium, supplied by Vitrolife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protective plustm  (Smiths Medical)
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Smiths Medical protective plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Protective Plustm, supplied by Smiths Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmdm hydantoin glydant plustm  (Lonza)
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Lonza dmdm hydantoin glydant plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Dmdm Hydantoin Glydant Plustm, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genjet plustm  (SignaGen)
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SignaGen genjet plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Genjet Plustm, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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securfit plustm  (Stryker)
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Stryker securfit plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Securfit Plustm, supplied by Stryker, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. Steadylite Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.

Journal: PLoS ONE

Article Title: A Novel High Throughput Invasion Screen Identifies Host Actin Regulators Required for Efficient Cell Entry by Toxoplasma gondii

doi: 10.1371/journal.pone.0064693

Figure Lengend Snippet: A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. Steadylite Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.

Article Snippet: Steadylite PlusTM (Promega) was dispensed onto the plates (10 μl/well), incubated at RT for 10 min and luminescence values reflecting parasite invasion were measured with the PHERstar plate reader.

Techniques: Transfection, Immunofluorescence, Western Blot, Lysis, Incubation, Luciferase, Activity Assay

A. Eighteen target genes represented in the library were silenced by siRNA transfection of HeLa cells in 96 well plates. The percent decrease in mRNA levels of target genes was determined using qRT-PCR 72 h post-transfection. n = 2 independent experiments, each with triplicate samples. Error bars, SEM. B. Schematic flowchart showing the siRNA screening strategy for identification of host factors playing a role in Toxoplasma invasion. The strategy consisted of three main steps of transfection, invasion and measurement. Host cell viability was assessed using CellTiter-Fluor™, which measures protease conversion of a quenched membrane permeable peptide substrate (glycine-phenylalanine-7-Amino-4-trifluormethylcoumarin). Parasite invasion was assessed using the luciferin-based reagent Steadylite Plus™, which measures the inducible luciferase activity of U-luc parasites.

Journal: PLoS ONE

Article Title: A Novel High Throughput Invasion Screen Identifies Host Actin Regulators Required for Efficient Cell Entry by Toxoplasma gondii

doi: 10.1371/journal.pone.0064693

Figure Lengend Snippet: A. Eighteen target genes represented in the library were silenced by siRNA transfection of HeLa cells in 96 well plates. The percent decrease in mRNA levels of target genes was determined using qRT-PCR 72 h post-transfection. n = 2 independent experiments, each with triplicate samples. Error bars, SEM. B. Schematic flowchart showing the siRNA screening strategy for identification of host factors playing a role in Toxoplasma invasion. The strategy consisted of three main steps of transfection, invasion and measurement. Host cell viability was assessed using CellTiter-Fluor™, which measures protease conversion of a quenched membrane permeable peptide substrate (glycine-phenylalanine-7-Amino-4-trifluormethylcoumarin). Parasite invasion was assessed using the luciferin-based reagent Steadylite Plus™, which measures the inducible luciferase activity of U-luc parasites.

Article Snippet: Steadylite PlusTM (Promega) was dispensed onto the plates (10 μl/well), incubated at RT for 10 min and luminescence values reflecting parasite invasion were measured with the PHERstar plate reader.

Techniques: Transfection, Quantitative RT-PCR, Membrane, Luciferase, Activity Assay